Little Mix stars Perrie Edwards, Leigh-Anne Pinnock and Jade Thirlwall appeared on Saturday night’s The Jonathan Ross.

They were noticeably missing their bandmate Jesse Nelson, who was taking some time off for personal reasons.

The stars haven’t said anything about Jesse’s absence and Jonathan has said he doesn’t want the Negev.

But he had some touching words for Jesse, telling the girls that he hopes you are fine.

Jonathan said, “It’s your tenth anniversary.”

Jade, Perry, and Lee-Ann didn’t respond to Jonathan’s words about Jesse, and they just clapped

“And before we talk about it, I just want to explain because there are three members of Little Mix here not four.

She released a press release this week to say that Jesse is taking time out for personal reasons.

“Obviously, I hope she knows this, and I hope she does, but everyone is hoping for her well and everyone hopes that she will get over it.”

Jonathan said he hopes troubled star Jesse will feel a lot better soon

He continued: “I will not intrude, I know it is completely personal, but everyone thinks, I hope you are fine.”

Jade, Berry, and Lee Anne smiled and applauded the audience, but did not comment further on Jesse’s condition.

One fan said, “Jonathan Ross wished Jesse the luck at the time was that warm guy.”

Jesy this week took a long break from Little Mix for medical reasons

Another added, “I miss Jesse and I hope you are well.”

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But as chatting turned into accents, the remaining Little Mix members impersonated some of their favorites.

Jesy is known for her brilliant talent and some fans thought it sad she wasn’t there because she would have loved the game.

One tweeted, “Jesse is the best in accents and it’s not there,” along with a sad face.

“Jesse is participating in the accent challenge at home # O LordAnother joked beside a photo of Jesse doing her infamous Jamaican accent.

Jesy’s announcement came after she had to miss several appearances with the band including the final of the talent show The Search on BBC One, as well as a party hosting them at the MTV European Music Awards.

She has raised concerns among fans after becoming noticeably absent on more than one of her notable Little Mix appearances in recent weeks.

A spokesperson confirmed: “Jesse is extending leave from Little Mix for special medical reasons.”

* The Jonathan Ross Show airs Friday Nights on ITV